Animal Cell Culture by Walker J.M. (ed.), Pollard J.W. (ed.)

By Walker J.M. (ed.), Pollard J.W. (ed.)

CIP cites the sequence because the major access. Covers equipment for developing mammalian fibroblastic mobile cultures and preserving tradition stipulations for epithelial, neuronal, and hematopoietic cells between others. recognition is given to the variety of tradition media and extracellular matrices had to hold the differential capabilities of the classy cells. additionally describes tradition suggestions for either residing and stuck cells.

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USA 70,190. Chapter 6 Scale-Up of Suspension and Anchorage-Dependent Animal Cells J, Bryan Griffiths 1. Introduction In this chapter, scale-up is described in a laboratory context (lo-20 L), but the principles and techniques employed have been successfully adapted so that cells are now grown industrially in unit volumes of up to 8000 L for vaccine, interferon, and monoclonal antibody production. The need to scale-up cell cultures has been expanded from the historical requirement for vaccine manufacture to include not only interferon and antibodies, but many important medical products such as tissue plasminogen activator and a range of hormones and blood factors.

2. Airlift-medium is circulated in a low velocity bulkflow pattern by being lifted up a central draft tube by rising air bubbles, and recirculated downwards in the outer ring formed by the draft tube. This system forms a near ideal mixing pattern and allows near-linear scale-up to at least 1000 L. Unfortunately, the apparatus, with a 121 aspect ratio, is very high and a 30-L fermenter needs 3-m ceiling height. , LH Fermentation ) or as disposable 570 mL U (CelliftFisher Scientific). 2. Culture Procedure for Suspension Cells 1.

Acad. Sci. 77,1885-1889. 4. Harley, C. B. and Goldstein, S. (1978) Cultured human fibroblasts: Distribution of cell generations and a critical limit. 1. CeEI. Physiol. 97,509-515. 5. Moerman, E. J. and Goldstein, S. (1986) Culture of human skin fibroblasts, Methods in Diabetes Research, Vol. II (Clarke, W. ), Wiley and Sons, New York, pp. 283-312. Chapter 4 Separation and Maintenance of Primary T and B Lymphocytes Derek Kinchington and Eleanor Berrie I. Introduction Two distinct populations of lymphocytes have been identified: T lymphocytes, which are thymus-dependent, and B cells, first observed in the Bursa Fabricus of birds.

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