By A. Leitmannova Liu
Advances in Planar Lipid Bilayers and Liposomes, quantity 6, maintains to incorporate invited chapters on a extensive variety of issues, masking either major preparations of the reconstituted process, particularly planar lipid bilayers and round liposomes. The invited authors current the most recent leads to this interesting multidisciplinary box in their personal examine group.
Many of the members operating in either fields over many a long time have been in shut collaboration with the past due Prof. H. Ti Tien, the founding editor of this publication sequence. There also are chapters written through the various more youthful new release of scientists integrated during this sequence. This quantity retains in brain the wider target with either structures, planar lipid bilayers and round liposomes, that is the extra improvement of this interdisciplinary box worldwide.
* Contributions from novices and demonstrated and skilled researchers
* Exploring theoretically and experimentally the planar lipid bilayer platforms and round liposomes
* This quantity is devoted to mark the Bilayer Lipid Membranes forty fifth anniversary
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Extra info for Advances in Planar Lipid Bilayers & Liposomes, Vol. 6
2. 5. 1. Addition of His-Tag to hSkM1 An advantage of the His-tag is its relatively short length compared to other available tags, which means that it is unlikely to affect function, thus removal of the tag is generally not necessary. The effect of additional amino acids on function is of particular concern for ion channels because small changes in structure often alter a channel’s functional properties. Many vectors are commercially available that contain His-tags at the C- or N-terminus, into which ion channel cDNA may be subcloned.
For protein production, cells were grown to a density of 2 Â 106 cells/ml, then infected with baculovirus (1–2 Â 107 PFU/ml). Protein production in Sf 9 cells infected with hSkM1-HT recombinant baculovirus was verified by electrophoresis and Western blot analysis using an anti-Pan Nav channel antibody (Alomone Labs, Jerusalem) (1:400), a secondary peroxidase conjugated goat anti-rabbit antibody (Amersham, England) (1:2000), and visualised using chemiluminesence (ECLTM, Amersham, England). Expression of hSkM1-HT in Sf 9 insect cells was confirmed by gel electrophoresis and immunodetection (Fig.
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