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1962) who had previously observed that certain basidiomycetes produced singles, pairs, or groups of polyacetylenes. ) QuB1. (family Tricholomataceae). The three diatretynes are shown below: HOOC -CH=CH-C C -C C -CONH, I HOOC-CH=CH-C=C-C=C-C=N I1 HOOC -CH=CH -C C -C C -C C- CH&H 111 20 ROBERT 6. BENEDICT The chromophores of diatretynes I, 11, and 111exhibit very characteristic UV absorption spectra. Additional supporting evidence for their presence was obtained by paper chromatography and in some cases by use of their bacterial spectra or behavior on countercurrent distribution, or both.